Invitrogen™

eBioscience™ Mouse Cytokine Positive Control Cells

Catalog number: 00-4500-51
Invitrogen™

eBioscience™ Mouse Cytokine Positive Control Cells

Catalog number: 00-4500-51
Catalog Number
00-4500-51
Unit Size
1 mL
Price (USD)
163.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
00-4500-511 mL
163.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
The Mouse Cytokine Positive Control Cells are recommended for use as positive control cells when establishing an intracellular cytokine staining protocol. Mouse splenocytes were stimulated with ConA (3 µg/mL) for two days, followed by recombinant IL-2 (20 ng/mL) and IL-4 (20 ng/mL) for three days. The cells were then restimulated with immobilized anti-mouse CD3 (10 µg/mL) and soluble anti-mouse CD28 (2 µg/mL) in the presence of Brefeldin A for 5 hours. These cells contain subpopulations that express IL-2, IL-3, IL-4, IL-10, GM-CSF, and IFNγ. The cells have been fixed with formaldehyde and are provided in a stabilizing buffer so that they may be stored at -80°C for up to six months or at 2-8°C for up to one month.

Reactivity/Species
Mouse

Reported Application
Intracellular Staining Followed by Flow Cytometric Analysis

Specifications

For Use With (Equipment)
Flow Cytometer
Product Line
eBioscience™
Product Type
Positive Control Cell

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