Membrane Blocking Solution can be used to block nitrocellulose and PVDF membranes, as well as to dilute antibodies or enzyme conjugates. This solution is a Tris buffer containing bovine serum albumin, goat IgG, Tween 20, and a mixture of other components that can reduce non-specific binding. The Proclin used in this reagent does not inhibit peroxidase or alkaline phosphatase activity. Note that this blocking solution contains normal goat IgG and should not be used in assays where anti-goat immunoglobulins are employed.