Anti-Clumping Agent This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: 0010057DG

Gibco™  Related applications: Antibody & Cell Culture Production | Mammalian Cell Culture

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Description

GIBCO® Anti-Clumping Agent is a concentrated liquid reagent that reduces cell clumping to assist in attaining higher viable cell densities in suspension culture. Anti-Clumping Agent is a gentle, animal origin-free, chemically defined formulation that contains no proteins, enzymes, hydrolysates, or components of unknown composition.

• Reduced cell clumping in suspension culture
• Higher viable cell densities
• Animal origin-free and chemically defined components
• Convenient packaging with a larger size now available
• Customization available to configure packaging and delivery

Simple and Gentle Cell Dissociation
Cell lines adapted to suspension culture can revert to a more adherent or aggregated “clumping" state. When this occurs, culture condition can be greatly improved by adding a gentle reagent to reduce clumping and adhesion instead of the harsh enzymes often found in cell dissociation products. Anti-Clumping Agent is a simple, gentle, concentrated solution which, when added to culture media (usually titrated between a 1:100 and 1:1000 dilution), can reduce cell clumping significantly.

Reduced Clumping, Increased Viability, Improved Protein Expression
Reduced cell clumping achieved through use of Anti-Clumping Agent is illustrated below (Fig 1). See also the data demonstrating improved viable cell density (Fig 2). Higher viable cell density contributes to the stabilization of culture conditions for better protein yield.

For actual cell dissociation, we recommend our animal origin-free TrypLE™ products.

Use Anti-Clumping Agent after Transfection
Anti-Clumping Agent is not recommended in all suspension cell culture conditions. For example, when creating a cell line using lipid cationic transfection, Anti-Clumping Agent can interfere with the transfection process, so in this case we recommend that Anti-Clumping Agent be used after transfection.

Animal Origin-free and Chemically Defined Components
Because Anti-Clumping Agent is made with only animal origin free and chemically defined components, you can be assured that the risk of adding adventitious agents to your culture system is minimized. Avoiding additives in your system that are not well defined can lead to more robust results from batch to batch.

Convenient and Customizable Packaging
Previously, Anti-Clumping Agent was only available in a 20 mL bottle. However, it is now available in a more economical and convenient 100 mL bottle. For even larger packaging, you can custom configure this product by clicking on the "Customize this Product" link under the "Add to Cart" button above or by emailing us at CustomMedia@lifetech.com.

Additional Information and Support
Please review the Manuals and Protocols provided below for additional information on successful use of this product. Direct any unanswered questions to our Technical Services group.

For Research Use Only. Not for use in diagnostics procedures.

Specifications

Classification: Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free
Reagent Type: Clumping Prevention Reagent
Form: Liquid
Format: Bottle(s)
Cell Line: CHO, 293 (HEK), Insect Cells
Culture Type: Suspension Cell Culture
Product Size: 100 mL
Volume (Metric): 100 ml
Green Features: Fewer resources used, Less waste
Shipping Condition: Room Temperature

Contents & storage

Store at 2 to 8°C

Documents

Manuals & protocols

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