123count eBeads are intended for use in absolute counting of cells or other particles by flow cytometry. These are 7 μm microparticles containing encapsulated dyes compatible with blue (488 nm) and violet (405 nm) excitation sources and emitting fluorescence between approximately 500 nm and 750 nm. They are supplied at a known concentration in 0.05% Tween-20 with 2 mM sodium azide. Collecting carefully suspended and measured quantities of cell:bead mixtures on a flow cytometer makes the ratiometric enumeration of cells possible.
Flow Cytometric Analysis