Achieve high-resolution separation of proteins and peptides under nondenaturing conditions with Thermo Scientific™ ProPac™ HIC-10 LC Columns. Developed with multi-functional ligand attachments, these silica-based columns provide exceptional hydrolytic stability under the highly aqueous conditions used in HIC.
Hydrophobic interaction chromatography separates biomolecules in a decreasing salt gradient, based on differences in surface hydrophobicity. This method preserves the biological activity of proteins. The HIC separation mechanism is complementary to those of ion-exchange and gel filtration chromatography.