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For high-resolution separations of free amino acids, choose the Thermo Scientific™ Dionex™ AminoPac™ PA10 Amino Acid Column. The Dionex AminoPac PA10 Column is packed with a hydrophobic, polymeric, pellicular, anion-exchange resin that is stable over the range of pH 0–14. The unique pH stability allows the use of eluents that are conducive to anodic oxidation of amino acids at gold electrodes. This column is recommended for use with the Thermo Scientific™ Dionex™ AAA-Direct™ Amino Acid Analyzer system, which allows direct detection of primary and secondary amino acids by integrated pulsed amperometry detection (IPAD) with no need for pre- or postcolumn derivatization.
Column Type | Guard Column |
Column Capacity | 12 µeq |
For Use With (Application) | Separation of Carbohydrates and Amino Acids |
For Use With (Equipment) | AAA-Direct System |
Solvent Compatibility | pH 0 to 14, 100% Compatible with Common Organic Solvents |
Stationary Phase | AminoPac PA10 |
Format | Microbore |
Temperature (Metric) | 30°C |
Length (Metric) | 50 mm |
Diameter (Metric) | 2 mm |
Unit Size | Each 1 |
Anion Exchange Capacity | 60 µeq/Column |
Column Construction | PEEK with 10-32 Threaded Ferrule-Style End Fittings. All Components Are Nonmetallic. |
Column Type | Analytical Column |
Crosslinking | 55% DVB |
Column Capacity | 60 µeq |
Flow Rate | 0.25 mL/min. |
For Use With (Application) | Separation of Carbohydrates and Amino Acids |
For Use With (Equipment) | AAA-Direct System |
Max. Pressure | 4000 psi (275 bar) |
Solvent Compatibility | pH 0 to 14, 100% Compatible with Common Organic Solvents, Sodium Acetate Ionic Form Eluents Only |
Particle Size | 8.5 µm |
Stationary Phase | AminoPac PA10 |
Format | Microbore |
Latex Crosslinking | 30 to 40% |
pH | 0 to 14 |
Pore Size | <10 Å |
Resin Composition | 8.5 µm Diameter Microporous (<10 Å) Bead (55% Cross-Linked) Agglomerated with an 80 nm Microbead Alkyl Ammonium Functionalized Latex (1% Cross-Linked). |
Solvents | 100% Compatible with Common Organic Solvents |
Temperature (Metric) | 30°C |
Temperature (Metric) Max | 40°C |
Length (Metric) | 250 mm |
Diameter (Metric) Latex | 180 nm |
Diameter (Metric) | 2 mm |
Unit Size | Each 1 |
Column Construction | PEEK with 10-32 Threaded Ferrule-Style End Fittings. All Components Are Nonmetallic. |
Column Type | Analytical Column |
Crosslinking | 55% DVB |
Column Capacity | 240 µeq |
Flow Rate | 0.25 mL/min. |
For Use With (Application) | Separation of Carbohydrates and Amino Acids |
For Use With (Equipment) | AAA-Direct System |
Max. Pressure | 4000 psi (275 bar) |
Solvent Compatibility | pH 0 to 14, 100% Compatible with Common Organic Solvents, Sodium Acetate Ionic Form Eluents Only |
Particle Size | 8.5 µm |
Stationary Phase | AminoPac PA10 |
Format | Standard Bore |
pH | 0 to 14 |
Pore Size | <10 Å |
Resin Composition | 8.5 µm Diameter Microporous (<10 Å) Bead (55% Cross-Linked) Agglomerated with an 80 nm Microbead Alkyl Ammonium Functionalized Latex (1% Cross-Linked). |
Solvents | 100% Compatible with Common Organic Solvents |
Temperature (Metric) | 30°C |
Temperature (Metric) Max | 40°C |
Length (Metric) | 250 mm |
Diameter (Metric) | 4 mm |
Unit Size | Each 1 |
Catalog Number | Specifications | Unit Size | Column Type | Diameter (Metric) | Length (Metric) | Price (USD) | Availability | Quantity | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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055407 | Each 1 | Guard Column | 2 mm | 50 mm | Price: 563.00 Your Price: | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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055406 | Each 1 | Analytical Column | 2 mm | 250 mm | Price: 1,630.00 Your Price: | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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SP5678 | Each 1 | Analytical Column | 4 mm | 250 mm | Price: 2,770.00 Your Price: | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The Dionex AAA-Direct system eliminates the need for any form of derivatization, and is therefore compatible with on-line monitoring of cell cultures and fermentation broths. Vitamins, amino sugars, and carbohydrates can be detected simultaneously with amino acids. Additional capabilities include separation of a wide range of sugars, phosphorylated amino acids, and common oxidation products of sulfur-containing amino acids, such as cysteic acid, methionine sulfone, and methionine sulfoxide.
Thermo Scientific offers two approaches to amino acid analysis: anion-exchange separation by the Dionex AAA-Direct system, in which amino acids are detected directly without the need for derivatization; and anion-exchange separation with postcolumn derivatization.
Dionex AminoPac columns are used for high-resolution separations of free amino acids as well as for separation and detection of a wide range of sugars, phosphorylated amino acids, and common oxidation products of sulfur-containing amino acids.