Sodium thiophosphate hydrate is used in thiosulfate-assisted synthesis of a variety of diamine-CuCN complexes. In analytical chemistry, it is used as a titrant in iodometry. It finds application as an anionic surfactant in pharmaceutical preparations to assist in dispersion and an antidote to cyanide poisoning. It is utilized for the dechlorination of tap water as well as to maintain the chlorine levels in swimming pools and aquaria. It plays an important role in gold extraction. It is also used in the alkylation reaction to prepare Bunte salts viz. S-alkylthiosulfonates, which finds application in the synthesis of thioglycolic acid.

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