Quickly and efficiently separate simple sugars without compromising resolution with Thermo Scientific™ Dionex CarboPac PA20 Columns. Combined with pulsed amperometric detection (PAD), these strong anion-exchange columns provide high-resolution separations at high pH with no need for derivatization. Achieve baseline resolution between the six monosaccharides commonly found in mammalian glycoproteins over a wide range of eluent concentrations.
High-Sensitivity Mono- and Disaccharide Analyses in Glycoprotein Therapeutics, Foods, and Beverages
Highly efficient separations with good resolution between neighboring peaks
Fast analysis times of less than 10 minutes
Lower eluent consumption
Optimized resolution of glucosamine/galactose and glucose/mannose peak pairs
Slightly different selectivity than the Dionex CarboPac PA1 or PA10 Columns; excellent for separating difficult peak pairs
Dionex CarboPac PA20 Columns consist of 6.5µm diameter nonporous beads covered with a fine latex of functionalized Thermo Scientific™ Dionex™ MicroBead™ resin. This pellicular resin structure permits excellent mass transfer, resulting in high-resolution chromatography and rapid re-equilibration.
Multiple Formats for Flexibility in Method Development
Dionex CarboPac PA20 Analytical column (3 x 150mm) provides high-resolution separations of mono- and disaccharides with no need for derivatization.
Dionex CarboPac PA20 Fast Sialic Acid column (3 x 30mm) accelerates sialic acid determination, allowing sample analysis in less than five minutes.
About the Dionex CarboPac Family of Columns The Dionex CarboPac family offers a selection of high-performance liquid chromatography (HPLC) columns, each optimized for a different class of compounds. Combined with PAD, which provides a means to detect carbohydrates without derivatization, these columns support simple, reliable techniques to separate sugar alcohols, mono- and disaccharides, and poly- and oligosaccharides.
Dionex CarboPac columns provide high-resolution separations of:
Glycoprotein oligosaccharides and complex carbohydrates from dietary fiber, including fructans, maltodextrins, and amylopectins
Monosaccharides, such as those obtained from glycoprotein hydrolysates or plant hydrolysates