For high-resolution mapping and analysis of oligosaccharides released from glycoproteins, use the Thermo Scientific™ Dionex™ CarboPac™ PA200 Analytical & Guard Columns to provide sensitive and specific detection. Combined with pulsed amperometric detection (PAD), this anion-exchange column provide fast, reproducible separations with no need for derivatization. The hydrophobic, polymeric, pellicular anion-exchange resin packing material is stable over the range of pH 0–14. This unique pH-stability allows the use of eluent compositions that are conducive to anodic oxidation of carbohydrates at gold electrodes.
Separations based on size, charge, degree of branching, and linkage isomerism
Low flow rate saves on eluent consumption
About the Dionex CarboPac Family of Columns
The Dionex CarboPac family offers a selection of high-performance liquid chromatography (HPLC) columns, each optimized for a different class of compounds. Combined with PAD, which provides a means to detect carbohydrates without derivatization, these columns support simple, reliable techniques to separate sugar alcohols, mono- and disaccharides, and poly- and oligosaccharides.
Dionex CarboPac columns provide high-resolution separations of:
Glycoprotein oligosaccharides and complex carbohydrates from dietary fiber, including fructans, maltodextrins, and amylopectins
Monosaccharides, such as those obtained from glycoprotein hydrolysates or plant hydrolysates
Mono- and disaccharides
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