Thermo Scientific™

ProPac™ IMAC-10 HPLC Columns

Catalog number: 063276
Have Questions?
Thermo Scientific™

ProPac™ IMAC-10 HPLC Columns

Catalog number: 063276
Have Questions?
Resolve target proteins using a single column in a high-resolution gradient run with Thermo Scientific™ ProPac™ IMAC-10 LC Columns. These analytical and semi-preparative HPLC columns provides high-purity separations of metal-binding proteins and high loading capacity for protein purification applications. The use of small, nonporous resin particles results in narrow, high-efficiency peaks, with minimal band broadening due to dispersion. Use loading columns to charge the ProPac IMAC-10 LC columns but avoid contact of the metal solution with the system.
 
Catalog Number
063276
Unit Size
Each
Particle Size
10 μm
Diameter (Metric)
4 mm
Length (Metric)
50 mm
Column Format
Analytical Column
Price (USD)
Full specifications
Quantity
Column FormatAnalytical Column
Diameter (Metric)4 mm
Flow Rate1 mL/min
For Use WithUltiMate 3000 HPLC System
Length (Metric)50 mm
Max. Pressure3000 psi (207 bar)
Mobile Phase CompatibilitypH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size10 μm
pH2 to 12
Pore SizeNonporous
Temperature60°C
Stationary PhaseLysozyne Poly(IDA) Grafts
Product LineProPac
Packing MaterialSpherical, Nonporous Polymer
Column TypeSeparation of Metal-Binding Proteins
PhaseAffinity Separation
TypeAnalytical Column
TechniqueBio-LC
Unit SizeEach
Showing 1 of 1
Catalog NumberSpecificationsUnit SizeParticle SizeDiameter (Metric)Length (Metric)Column FormatPrice (USD)Quantity
063276Full specifications
Each 10 μm4 mm50 mmAnalytical Column
Column FormatAnalytical Column
Diameter (Metric)4 mm
Flow Rate1 mL/min
For Use WithUltiMate 3000 HPLC System
Length (Metric)50 mm
Max. Pressure3000 psi (207 bar)
Mobile Phase CompatibilitypH 2 to 12, compatible with most reagents used in IMAC protein purifications, including EtOH, Urea, NaCl, non-ionic detergents, glycerol, acetic acid, guanidine, HCl
Particle Size10 μm
pH2 to 12
Pore SizeNonporous
Temperature60°C
Stationary PhaseLysozyne Poly(IDA) Grafts
Product LineProPac
Packing MaterialSpherical, Nonporous Polymer
Column TypeSeparation of Metal-Binding Proteins
PhaseAffinity Separation
TypeAnalytical Column
TechniqueBio-LC
Unit SizeEach
Showing 1 of 1
Tailored Specificity for IMAC Separation of Proteins
  • Retention control by imidazole concentration or pH gradient
  • Stable between pH 2 to 12, compatible with common protein purification reagents (nonionic detergents, reducing agents)
  • Effective under native or denaturing HPLC conditions
  • Wide range of metal-specific applications, including the separation of His-tagged protein aggregation variants, prion peptide analysis and separation of monoclonal antibodies
  • Shipped free from any metal and ready to be charged with the metal of your choice.
  • Reusable columns with metal tailored specificity
  • Resolve target proteins using a single column in a high resolution gradient run
  • High purity separations of metal-binding proteins
Includes: 10μm, nonporous, polymeric beads coated with a hydrophilic layer, then grafted with poly(IDA) chains.

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