Thermo Scientific™

pH Gradient Buffers

Catalog number: 085346
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Thermo Scientific™

pH Gradient Buffers

Catalog number: 085346
Have Questions?

Obtain fast and highly robust, reproducible HPLC gradients using Thermo Scientific™ CX-1 pH Gradient Buffers. Simple to optimize and easily automated, these buffers are applicable for HPLC separation of the majority of monoclonal antibody (mAb) biotherapeutics drugs. CX-1 pH Gradient Buffers are ready to use with existing HPLC columns without the need to formulate mobile phases. The CX-1 buffers save time in method development, facilitate method transfer to QA/QC for a wide range of mAb charge variants through a generic HPLC-based approach to mAb characterization, and do not require time-consuming mobile phase adjustments.

 
Catalog Number
085346
Unit Size
Each
Description
CX-1 pH Gradient Buffer A
pH
5.6
Quantity
250 mL
Price (USD)
Full specifications
Quantity
DescriptionCX-1 pH Gradient Buffer A
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
TypeCX-1 pH Gradient Buffer A
Volume (Metric)250 mL
pH5.6
Quantity250 mL
Unit SizeEach
Catalog Number
Unit Size
Each
Description
CX-1 pH Gradient Buffer A
pH
5.6
Quantity
125 mL
Price (USD)
Full specifications
Quantity
DescriptionCX-1 pH Gradient Buffer A
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
TypeCX-1 pH Gradient Buffer A
Volume (Metric)125 mL
pH5.6
Quantity125 mL
Unit SizeEach
Catalog Number
Unit Size
Each
Description
CX-1 pH Gradient Buffer B
pH
10.2
Quantity
125 mL
Price (USD)
Full specifications
Quantity
DescriptionCX-1 pH Gradient Buffer B
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
TypeCX-1 pH Gradient Buffer B
Volume (Metric)125 mL
pH10.2
Quantity125 mL
Unit SizeEach
Catalog Number
Unit Size
Each
Description
CX-1 pH Gradient Buffer B
pH
10.2
Quantity
250 mL
Price (USD)
Full specifications
Quantity
DescriptionCX-1 pH Gradient Buffer B
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
TypeCX-1 pH Gradient Buffer B
Volume (Metric)250 mL
pH10.2
Quantity250 mL
Unit SizeEach
Catalog Number
Unit Size
Each
Description
CX-1 pH Gradient Buffer A
pH
5.6
Quantity
500 mL
Price (USD)
Full specifications
Quantity
DescriptionCX-1 pH Gradient Buffer A
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
TypeCX-1 pH Gradient Buffer A
pH5.6
Quantity500 mL
Unit SizeEach
Catalog Number
Unit Size
Each
Description
CX-1 pH Gradient Buffer B
pH
10.2
Quantity
500 mL
Price (USD)
Full specifications
Quantity
DescriptionCX-1 pH Gradient Buffer B
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
TypeCX-1 pH Gradient Buffer B
pH10.2
Quantity500 mL
Unit SizeEach
Catalog Number
Unit Size
Each
Description
CX-1 pH Gradient Buffer A
pH
5.6
Quantity
1 L
Price (USD)
Full specifications
Quantity
DescriptionCX-1 pH Gradient Buffer A
For Use With (Equipment)HPLC Columns Including ProPac Elite WCX and MAbPac SCX HPLC Columns
Product LineThermo Scientific
TypeCX-1 pH Gradient Buffer A
Volume (Metric)1000 mL
pH5.6
Quantity1 L
Unit SizeEach
Catalog Number
Unit Size
Each
Description
CX-1 pH Gradient Buffer B
pH
10.2
Quantity
1 L
Price (USD)
Full specifications
Quantity
DescriptionCX-1 pH Gradient Buffer B
For Use With (Equipment)HPLC Columns Including ProPac Elite WCX and MAbPac SCX HPLC Columns
Product LineThermo Scientific
TypeCX-1 pH Gradient Buffer B
Volume (Metric)1000 mL
pH10.2
Quantity1 L
Unit SizeEach
Showing 8 of 8
Catalog NumberSpecificationsUnit SizeDescriptionpHQuantityPrice (USD)Quantity
085346Full specifications
EachCX-1 pH Gradient Buffer A5.6250 mL
DescriptionCX-1 pH Gradient Buffer A
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
TypeCX-1 pH Gradient Buffer A
Volume (Metric)250 mL
pH5.6
Quantity250 mL
Unit SizeEach
083273Full specifications
EachCX-1 pH Gradient Buffer A5.6125 mL
DescriptionCX-1 pH Gradient Buffer A
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
TypeCX-1 pH Gradient Buffer A
Volume (Metric)125 mL
pH5.6
Quantity125 mL
Unit SizeEach
083275Full specifications
EachCX-1 pH Gradient Buffer B10.2125 mL
DescriptionCX-1 pH Gradient Buffer B
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
TypeCX-1 pH Gradient Buffer B
Volume (Metric)125 mL
pH10.2
Quantity125 mL
Unit SizeEach
085348Full specifications
EachCX-1 pH Gradient Buffer B10.2250 mL
DescriptionCX-1 pH Gradient Buffer B
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
TypeCX-1 pH Gradient Buffer B
Volume (Metric)250 mL
pH10.2
Quantity250 mL
Unit SizeEach
302779Full specifications
EachCX-1 pH Gradient Buffer A5.6500 mL
DescriptionCX-1 pH Gradient Buffer A
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
TypeCX-1 pH Gradient Buffer A
pH5.6
Quantity500 mL
Unit SizeEach
302780Full specifications
EachCX-1 pH Gradient Buffer B10.2500 mL
DescriptionCX-1 pH Gradient Buffer B
For Use With (Equipment)LC Columns and Systems
Product LineThermo Scientific
TypeCX-1 pH Gradient Buffer B
pH10.2
Quantity500 mL
Unit SizeEach
303274Full specifications
EachCX-1 pH Gradient Buffer A5.61 L
DescriptionCX-1 pH Gradient Buffer A
For Use With (Equipment)HPLC Columns Including ProPac Elite WCX and MAbPac SCX HPLC Columns
Product LineThermo Scientific
TypeCX-1 pH Gradient Buffer A
Volume (Metric)1000 mL
pH5.6
Quantity1 L
Unit SizeEach
303275Full specifications
EachCX-1 pH Gradient Buffer B10.21 L
DescriptionCX-1 pH Gradient Buffer B
For Use With (Equipment)HPLC Columns Including ProPac Elite WCX and MAbPac SCX HPLC Columns
Product LineThermo Scientific
TypeCX-1 pH Gradient Buffer B
Volume (Metric)1000 mL
pH10.2
Quantity1 L
Unit SizeEach
Showing 8 of 8

pH buffer solutions can be used to generate highly reproducible, linear pH gradients in cation exchange chromatography.

  • Robust, reproducible gradients
  • Predict biomolecule pH
  • Simple method optimization
  • Easily automated
  • Applicable to the majority of MAbs

Unlike traditional cation exchange chromatography using salt gradients, it is possible to predict the pH and the expected retention of the charge variants and use a narrow pH range to get a higher resolution separation.

WARNING: Cancer and Reproductive Harm – www.P65Warnings.ca.gov

Thermo Scientific pH buffer concentrates used in the pH gradient platform can be purchased individually or as a pair, in quantities of 125mL or 250mL. For added convenience, the 125mL buffers can also be bundled with columns in a number of specifically preconfigured kits.

  • The CX-1 pH gradient starter kit contains 125mL each of buffers A and B, plus a MAbPac SCX-10, 10μm, 4 x 250mm column
  • The CX-1 pH gradient high throughput kit contains 125mL each of buffers A and B, plus a MAbPac SCX-10, 5μm, 4 x 50mm column
  • The CX-1 pH gradient high resolution kit contains 125mL each of buffers A and B, plus a MAbPac SCX-10, 5μm, 4 x 250mm column

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