EGFR Recombinant Human Protein (without Catalytic Activity Domain), His Tag, Insect cell expressed

Catalog number: 10001H08B50

 Related applications: Protein Assays and Analysis

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Description

Epidermal growth factor receptor (EGFR), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

Since this product is a truncated protein and does not contain intracellular catalytic activity domain, it is not suitable for use in enzyme activity studies.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Lys 642) of human EGFR (NP_005219) fused to a polyhistidine tag at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA. Immobilized human EGF at 10 µg/ml can bind human EGFR with a linear range of 3.2-400 ng/ml.

Formulation: Lyophilized in 20 mM Tris, pH7.4, 50 mM NaCl, 3 mM DTT, 0.5 mM TCEP, 5% Mannitol, 5% Trehalose, 0.02% Tween-80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by SINO Biological.

Specifications

Protein Family: Growth Factors & Receptors
Research Category: Cardiovascular Research, Oncology, Signal Transduction, Stem Cell Research
Endotoxin Level: < 1 EU/µg
Expression: Insect
Form: Lyophilized
Gene Aliases: EGFR, ErbB, HER1, mENA, ErbB1, PIG61
Gene Symbol: EGFR
Kinase Family: EGF Receptor (ErbB) Kinase Family
Molecular Weight: 69.2 kDa
Product Line: Sino Biological®
Product Size: 50 µg
Protein Form: Cell Surface Protein, Recombinant, Truncated
Protein Name: Epidermal growth factor receptor
Protein Subtype: Growth Factor Receptors, Receptor Tyrosine Kinases
Protein Tag or Fusion: His Tag
Purity: > 95% by SDS-PAGE
Species: Human
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols

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