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NRP1 Recombinant Human Protein, His Tag

Catalog number: 10011H08H50

 Related applications: Protein Assays and Analysis

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Description

Neutropilin-1 (NRP1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the human NRP1 isoform b (NP_001019799.1) (Met 1-Lys 644) fused to the polyhistidine tag at the C-terminus.

Activity: Measured by using the Octet RED System, the affinity constant (Kd) of NRP1-His bound to biotinylated human VEGF165 was 25 nM.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: RP11-342D11.1, BDCA4, CD304, DKFZp686A03134, DKFZp781F1414,NP1, NRP1, VEGF165R
Gene Symbol: NRP1
Molecular Weight: 71.3 kDa
Product Size: 50 µg
Protein Family: Growth Factors & Receptors, Other Proteins
Protein Subtype: Growth Factor Receptors
Protein Tag or Fusion: His Tag
Purity: ≥ 90% by SDS-PAGE
Research Category: Angiogenesis, Immunology, Oncology
Species: Human
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols

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