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KDR (CD309) Recombinant Human Protein (without Catalytic Activity Domain), hIgG1-Fc Tag

Catalog number: 10012H02H5

 Related applications: Protein Assays and Analysis

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Description

Kinase insert domain receptor (KDR/CD309), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in studying protein-protein interactions, and cell biology research applications. It can also be used as a protein standard.

Since this product is a truncated protein and does not contain intracellular catalytic activity domain, it is not suitable for use in enzyme activity studies.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Glu 764) of human KDR (NP_002244.1) fused to the Fc region of human IgG1 at the C-terminus.

Activity:
1. Measured by its binding ability in a functional ELISA. Immobilized recombinant human VEGFA at 1.25 µg/ml (100 µl/well) can bind KDR with a linear range of 1.25-40.0 ng/ml.
2. Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 9.6 ng/mL in the presence of 5-20 ng/mL recombinant human VEGF165.

Formulation: Lyophilized in PBS, pH7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween-80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by SINO Biological.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: CD Proteins, Growth Factors & Receptors
Research Category: Cardiovascular Research, Immunology, Oncology, Stem Cell Research
Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: CD309, FLK1, VEGFR, VEGFR2, KDR
Gene ID: 3791
Gene Symbol: KDR
Kinase Family: VEGF Receptors
Molecular Weight: 109 kDa
Product Line: Sino Biological®
Product Size: 5 µg
Protein Form: Recombinant, Truncated
Protein Name: kinase insert domain receptor (a type III receptor tyrosine kinase)
Protein Subtype: Growth Factor Receptors, Receptor Tyrosine Kinases
Protein Tag or Fusion: IgG1 Fc Tag
Purity: ≥ 85% by SDS-PAGE
Species: Human
Validated Application: Assay Standard

Contents & storage

Store at -20 to -70 °C

Documents

Manuals & protocols

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