Fetal Bovine Serum, certified, heat inactivated, United States

***ATTENTION***

*** NEW CATALOG NUMBER***



These products are being replaced with Premium Plus (FBS) performance-based products. The Premium Plus product specifications are improved and product stability, performance, manufacturing, storage, and transport processes are unchanged.

New and improved replacement available, Buy now›
Fetal Bovine Serum, certified, heat inactivated, United States
Gibco™

Fetal Bovine Serum, certified, heat inactivated, United States

Important Update: We've recently introduced Premium Plus Fetal Bovine Serum (FBS) specification-based products. These improved products are alternatives to our Certified US origin, New Zealand origin, and Qualified Australia origin SKUs. Product stability, performance, manufacturing, storage, and transport processes are unchanged.
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Catalog NumberQuantityFormat
10082147500 mLBottle
A384000150 mLOne Shot
A384000210 x 50 mLOne Shot
Our improved Premium Plus Fetal Bovine Serum (FBS) features enhanced specifications with unchanged stability, manufacturing, storage, and transport processes.
Catalog number 10082147
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Price (USD)
1,076.00
Each
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Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements

  • Sera Category: Premium (Performance Plus)
  • Origin: United States
  • Endotoxin level: ≤5 EU/mL
  • Hemoglobin level: ≤15 mg/dL
For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.
Specifications
Endotoxin Concentration≤5 EU/mL
Hemoglobin Concentration≤15 mg/dL
Purity or Quality GradeCertified
Shipping ConditionFrozen
SpeciesCattle/Bovine
AgeFetal
Country of OriginUnited States
FormLiquid
FormatBottle
Product TypeFetal Bovine Serum
Quantity500 mL
Serum TreatmentHeat-inactivated
Unit SizeEach
Contents & Storage
Storage conditions: ≤-10°C
Shipping conditions: Frozen

Frequently asked questions (FAQs)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How much of the total protein measured in Fetal Bovine Serum (heat inactivated or otherwise) is attributed to albumin?

Our CoAs only capture the total protein content not specifically the amount attributed to albumin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How much Fetal Bovine Serum (FBS) do I need to add to my medium?

FBS is added to culture medium at a concentration of 2-10% to provide attachment factors, nutrients, and hormones for mammalian cells, as well as to be a buffer against disruptions like pH changes and endotoxins. FBS has significant amounts of embryonic growth promoting factors like hormones, carrier proteins, and macromolecular proteins. It also has low levels of antibodies and other growth-inhibiting components. For most basal medium, 10% FBS is used. Some applications require up to 20% supplementation, so researchers need to determine what is optimal for their specific application and cell line.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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