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Fetal Bovine Serum, 적합(qualified), 호주
Fetal Bovine Serum, 적합(qualified), 호주
인용 및 참조 문헌 (1)
Gibco™

Fetal Bovine Serum, 적합(qualified), 호주

Gibco fetal bovine sera는 기본 세포 배양, 특수 연구 및 특정 분석에 뛰어난 가치를 제공하며, 연구자의 연구 요구 사항과 예산자세히 알아보기
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카탈로그 번호수량형식순도 또는 품질 등급
10099141500 mL병적합(Qualified)
Premium Plus Fetal Bovine Serum (FBS) 은 안정성 및 제조 공정은 그대로 유지되면서 품질이 더욱 강화되었습니다.
카탈로그 번호 10099141
현재 제품 선택
Gibco fetal bovine sera는 기본 세포 배양, 특수 연구 및 특정 분석에 뛰어난 가치를 제공하며, 연구자의 연구 요구 사항과 예산 요구 사항을 충족할 수 있도록 일관된 품질 및 우수성을 널리 인정받은 지원을 통해 신뢰할 수 있는 결과를 제공합니다.

혈청 카테고리: Premium(Secure)
원산지: 호주.
엔도톡신 함량: <=5 EU/mL.
헤모글로빈 함량: <=30 mg/dL (일반 수준 <=25 mg/dL).
For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.
사양
엔도톡신 농도≤5 EU/mL
헤모글로빈 농도≤30 mg/dL
순도 또는 품질 등급적합(Qualified)
배송 조건냉동 상태
종소
Age태아
원산지호주
형태액체
형식병
제품 유형소 태아 혈청(Fetal Bovine Serum)
수량500 mL
Serum TreatmentStandard (Sterile-Filtered)
멸균멸균
Unit SizeEach
구성 및 보관
보관 조건: ≤-10°C
배송 조건: 냉동 상태

자주 묻는 질문(FAQ)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I minimize lot-to-lot variability in serum?

You can test each new lot to be sure that it works for your specific applications. Alternatively, our serum sales team can determine the most consistent, highest-performing serum lot available using the Gibco iMatch Sera Lot Matching Tool.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I aliquot and refreeze serum?

Yes, after the overnight thaw, you can aliquot the serum into smaller, single-use aliquots and refreeze it.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Fetal Bovine Serum, 적합(qualified), 호주 FAQs

인용 및 참조 문헌 (1)

인용 및 참조 문헌
Abstract
Lack of Fucose on Human IgG1 N-Linked Oligosaccharide Improves Binding to Human Fcgamma RIII and Antibody-dependent Cellular Toxicity.
Authors: Shields Robert L; Lai Jadine; Keck Rodney; O'Connell Lori Y; Hong Kyu; Meng Y Gloria; Weikert Stefanie H A; Presta Leonard G;
Journal:J Biol Chem
PubMed ID:11986321
Lec13 cells, a variant Chinese hamster ovary cell line, were used to produce human IgG1 that were deficient in fucose attached to the Asn(297)-linked carbohydrate but were otherwise similar to that found in IgG1 produced in normal Chinese hamster ovary cell lines and from human serum. Lack of fucose on ... More