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CD34 Recombinant Human Protein, hIgG1-Fc tag

Catalog number: 10103H02H50

 Related applications: Protein Assays and Analysis

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Description

Cluster of Differentiation 34 (CD34), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in performing cell based assays. It can also be used as a protein standard, or in other research applications.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of the human CD34 precursor (NP_001020280.1) (Met 1-Thr 290) fused to the Fc region of human IgG1 at the C-terminus.

Activity: Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line . When 4 x 104 cells/well are added to human CD34 coated plates (100 µl/well of a 0.8 µg/mlsolution), >40 % will adhere after one hour at 37°C.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: CD Proteins
Research Category: Immunology, Signal Transduction
Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: RP11-328D5.2, CD34
Gene Symbol: CD34
Molecular Weight: 54 kDa
Product Line: Sino Biological®
Product Size: 50 µg
Protein Form: Cell Surface Protein, Recombinant, Truncated
Protein Name: CD34 molecule
Protein Subtype: Glycoproteins
Protein Tag or Fusion: IgG1 Fc Tag
Purity: ≥ 90% by SDS-PAGE
Species: Human
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols