CD34 Recombinant Human Protein, His Tag

Catalog number:  10103H08H50

 Related applications: Protein Assays and Analysis

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Description

CD34 Molecule (CD34), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in performing cell-based assays and protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human CD34 precursor (NP_001020280.1) (Met 1-Thr 290) fused to a polyhistidine tag at the C-terminus.

Activity:
1. Measured by its ability to bind human CD62L in a functional ELISA.
2. Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line. When 4 x 104 cells/well are added to human CD34 coated plates (0.8 µg/ml, 100 µl/well), approximately >40 % will adhere after one hour at 37°C.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 25 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by SINO Biological.

Specifications

Protein Family: CD Proteins
Research Category: Inflammation, Oncology, Stem Cell Research
Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: RP11-328D5.2, CD34
Gene Symbol: CD34
Molecular Weight: 29 kDa
Product Line: Sino Biological®
Product Size: 50 µg
Protein Name: CD34 molecule
Protein Subtype: Other Proteins
Protein Tag or Fusion: His Tag
Purity: ≥ 90% by SDS-PAGE
Species: Human
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols