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VCAM1 Recombinant Human Protein (N-term His Tag)

Catalog number: 10113H07H50

 Related applications: Protein Assays and Analysis

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Description

Vascular cell adhesion molecule 1 (VCAM1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human VCAM1 (NP_001069.1) (Phe 25 - Pro 697) fused to a polyhistidine tag at the N-terminus.

Activity: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When cells are added to VCAM1 coated plates ( 10 µg/ml, 100 µl/well ) approximately 85-95% will adhere specifically

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: CD Proteins
Research Category: Angiogenesis, Extracellular Matrix & Cell Adhesion, Immunology, Inflammation
Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: VCAM1, CD106, DKFZp779G2333, INCAM-100, MGC99561
Gene Symbol: VCAM1
Molecular Weight: 76 kDa
Product Line: Sino Biological®
Product Size: 50 µg
Protein Form: Cell Surface Protein, Recombinant, Truncated
Protein Name: vascular cell adhesion molecule 1
Protein Subtype: Other Proteins
Protein Tag or Fusion: His Tag
Purity: ≥ 90% by SDS-PAGE
Species: Human
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols