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AMICA1 (JAML) Recombinant Human Protein, His tag

Catalog number:  10120H08H5

 Related applications: Protein Assays and Analysis

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Description

Adhesion molecule, interacts with CXADR antigen 1 (AMICA1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human AMICA1 (NP_001091996.1) (Met 1-Leu 275) fused to a polyhistidine tag at the C-terminus.

Activity: Measured by its ability to inhibit NGF-induced proliferation of TF1 human erythroleukemic cells
. When 5 x 104 cells/well are added to AMICA1 coated plates (10 µg/ml and 100 µL/well), approximately 32% will adhere specifically after 60 minutes at 37°C.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: JAML, AMICA, Gm638, CREA7-1, CREA7-4, FLJ37080, MGC118814,MGC118815, AMICA1
Gene Symbol: AMICA1
Molecular Weight: 30.5 kDa
Product Line: Sino Biological®
Product Size: 5 µg
Protein Family: Other Proteins
Protein Name: adhesion molecule, interacts with CXADR antigen 1
Protein Subtype: Intercellular adhesion Molecule
Protein Tag or Fusion: His Tag
Purity: ≥ 85% by SDS-PAGE
Research Category: Oncology
Species: Human
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols