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FLT1 (VEGFR1) Recombinant Human Protein (without Catalytic Activity Domain), His Tag

Catalog number: 10136H08H250

 Related applications: Protein Assays and Analysis

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Fms-related tyrosine kinase 1 (FLT1 /VEGFR1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

Since this product is a truncated protein and does not contain intracellular catalytic activity domain, it is not suitable for use in enzyme activity studies.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Asn 756) of human FLT1 (P17948-1) fused to a polyhistidine tag at the C-terminus.

Activity: Measured by its ability to inhibit the VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC) (Conn, G. et al.,1990 , Proc. Natl. Acad. Sci. USA 87:1323). The ED50 for this effect is typically 5-30 ng/ml in the presence of 5 ng/ml human VEGF165.

Formulation: Lyophilized in PBS, pH7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween-80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by SINO Biological.

For Research Use Only. Not for use in diagnostic procedures.


Form: Lyophilized
Purity: ≥ 85% by SDS-PAGE
Gene ID: 2321
Species: Human
Expression: Mammalian
Gene Symbol: FLT1
Gene Aliases: FLT, VEGFR1, FLT1
Product Size: 5 x 50 µg
Protein Form: Truncated, Recombinant
Kinase Family: VEGF Receptors
Protein Family: Growth Factors & Receptors
Endotoxin Level: < 1 EU/µg
Protein Subtype: Growth Factor Receptors, Receptor Tyrosine Kinases
Molecular Weight: 83.7 kDa
Research Category: Signal Transduction, Cardiovascular Research, Oncology, Stem Cell Research
Protein Tag or Fusion: His Tag
Validated Application: Assay Standard
Shipping Condition: Room Temperature

Contents & storage

Store at -20 to -70 °C


Manuals & protocols

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