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IL1B Recombinant Human Protein, N-His Tag

Catalog number: 10139H07E50

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Interleukin 1, beta (IL1B), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the proform of human IL1B (NP_000567.1) (Met 1-Ser 269) fused to a polyhistidine tag at the N-terminus.

Activity: Measured by its binding ability in a functional ELISA. Immobilized human IL1B at 10 µg/ml (100 µl/well) can bind human IL1R1. The EC50 of human IL1R1 is 0.198 µg/ml.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.


Form: Lyophilized
Purity: ≥ 90% by SDS-PAGE
Species: Human
Expression: E. coli
Gene Symbol: IL1B
Gene Aliases: IL-1, IL1-BETA, IL1F2, IL1B
Product Size: 50 µg
Protein Family: Cytokines & Receptors
Protein Subtype: Interleukins
Molecular Weight: 32.3 kDa
Research Category: Immunology, Cell Cycle & Proliferation
Protein Tag or Fusion: His Tag
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.


Manuals & protocols

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