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IL1B Recombinant Human Protein, N-His Tag

Catalog number: 10139H07E50

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Description

Interleukin 1, beta (IL1B), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the proform of human IL1B (NP_000567.1) (Met 1-Ser 269) fused to a polyhistidine tag at the N-terminus.

Activity: Measured by its binding ability in a functional ELISA. Immobilized human IL1B at 10 µg/ml (100 µl/well) can bind human IL1R1. The EC50 of human IL1R1 is 0.198 µg/ml.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Research Category: Cell Cycle & Proliferation, Immunology
Expression: E. coli
Form: Lyophilized
Gene Aliases: IL-1, IL1-BETA, IL1F2, IL1B
Gene Symbol: IL1B
Molecular Weight: 32.3 kDa
Product Line: Sino Biological®
Product Size: 50 µg
Protein Family: Cytokines & Receptors
Protein Name: interleukin 1, beta
Protein Subtype: Interleukins
Protein Tag or Fusion: His Tag
Purity: ≥ 90% by SDS-PAGE
Species: Human
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols

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