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LEP (Leptin) Recombinant Human Protein

Catalog number: 10221HNAE5

 Related applications: Protein Assays and Analysis

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Description

Leptin (LEP), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the mature form of human Leptin (NP_000221.1), residues 22 to 167, containing an additional Met.

Activity: Measured by its binding ability in a functional ELISA
1. Immobilized human Leptin at 1.25 µg/ml(100 µl/well) can bind human Leptin receptor-Fc with a linear range of 0.032-4.0 µg/ml.
2. Immobilized human Leptin at 5 µg/ml(100 µl/well) can bind human Leptin receptor-His with a linear range of 0.032-4.0 µg/ml.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Research Category: Other Research, Signal Transduction
Expression: E. coli
Form: Lyophilized
Gene Aliases: FLJ94114, OB, OBS
Gene ID: 3952
Gene Symbol: LEP
Molecular Weight: 16 kDa
Product Line: Sino Biological®
Product Size: 5 µg
Protein Family: Other Proteins
Protein Form: Native
Protein Name: Leptin
Protein Subtype: Lectins & Leptins
Protein Tag or Fusion: Untagged
Purity: ≥ 95% by SDS-PAGE
Species: Human
Validated Application: Assay Standard

Contents & storage

Store at -20 to -70 °C

Documents

Manuals & protocols

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