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CD40LG Recombinant Human Protein, hIgG1-Fc Tag

Catalog number: 10239H01H25

 Related applications: Protein Assays and Analysis

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CD40 ligand (CD40LG), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as protein structure analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the soluble form of human CD40LG (NP_000065.1) (Met 113-Leu 261) fused to the Fc region of human IgG1 at the N-terminus.

Activity: Measured by its binding ability in a functional ELISA
. Immobilized human CD40 at 2 µg/ml (100 µl/well) can bind human CD40LG-Fc chimera with a linear range of 1.28-32 ng/ml.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.


Form: Lyophilized
Purity: ≥ 85% by SDS-PAGE
Species: Human
Expression: Mammalian
Gene Symbol: CD40LG
Gene Aliases: RP3-527F8.3, CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L
Product Size: 5 x 5 µg
Protein Family: CD Proteins
Endotoxin Level: < 1 EU/µg
Protein Subtype: TNF (Tumor Necrosis Factor)
Molecular Weight: 42.8 kDa
Research Category: Immunology, Signal Transduction
Protein Tag or Fusion: IgG1 Fc Tag
Validated Application: Assay Standard

Contents & storage

Includes 5 vial of lyophilized protein. Store at -20 °C.


Manuals & protocols