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FCGR1A (CD64) Recombinant Human Protein, His Tag

Catalog number: 10256H08H5

 Related applications: Protein Assays and Analysis

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Description

Fc gamma receptor I-a (FCGR1A/CD64), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, studying protein-protein interactions, and cell biology research applications. It can also be used as an immunogen, or as a protein standard.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Pro 288) of human FCGR1A (NP_000557.1) fused to a polyhistidine tag at the C-terminus.

Activity: Measured by its binding ability in a functional ELISA
1. Immobilized CD64 at 10 µg/mlcan bind human IgG1 with a linear range of 0.39-25 ng/ml.
2. Scatchard analysis showed that the affinity constant (Kd) of CD64 bound to human IgG1 was 0.059 nM .

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: CD Proteins, Growth Factors & Receptors
Research Category: Apoptosis, Immunology, Oncology, Signal Transduction
Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: FCGR1A, CD64, CD64A, FCRI, FLJ18345, IGFR1
Gene ID: 2209
Gene Symbol: FCGR1A
Molecular Weight: 32 kDa
Product Line: Sino Biological®
Product Size: 5 µg
Protein Form: Cell Surface Protein, Recombinant, Truncated
Protein Name: Fc fragment of IgG, high affinity Ia, receptor (CD64)
Protein Subtype: Fc Receptors, Glycoproteins
Protein Tag or Fusion: His Tag
Purity: > 85% by SDS-PAGE
Species: Human
Validated Application: Assay Standard

Contents & storage

Store at -20 to -70 °C

Documents

Manuals & protocols