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PTPN1 Recombinant Human Protein, N-His Tag

Catalog number: 10304H07E250

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Description

Protein tyrosine phosphatase, non-receptor type 1 (PTPN1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the human PTPN1 (NP_002818.1) (Glu 2 -Asn 321) fused to a polyhistidine tag at the N-terminus.

Activity: Measured by its ability to dephosphorylate a phosphotyrosine residue in an EGF receptor (aa988-998) phosphopeptide substrate. The specific activity is>15 nmoles/min/µg.

Formulation: Lyophilized in 10 mM HEPES, 500 mM NaCl, 1 mM DTT, 1 mM EDTA, pH 7.5, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: Other Proteins
Research Category: Drug Development, Other Research
Expression: E. coli
Form: Lyophilized
Gene Aliases: PTP1B, PTPN1
Gene Symbol: PTPN1
Molecular Weight: 38 kDa
Product Line: Sino Biological®
Product Size: 5 x 50 µg
Protein Name: protein tyrosine phosphatase, non-receptor type 3
Protein Subtype: Other Proteins
Protein Tag or Fusion: His Tag
Purity: ≥ 95% by SDS-PAGE
Species: Human
Validated Application: Assay Standard

Contents & storage

Includes 5 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols