Interested in a bulk or custom version of this product? Request pricing.

SERPINA1 Recombinant Human Protein, His Tag

Catalog number: 10306H08H25

 Related applications: Protein Assays and Analysis

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

Serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1 (SERPINA1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the human SERPINA1 pre-protein (NP_000286.3) (Met 1-Lys 418) fused to the polyhistidine tag at the C-terminus.

Activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2
. The IC50 value is<3.0 nM, as measured in 100 µl reaction mixture containing 1.25 ng trypsin, 10 µM substrate, 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, pH 7.5.

Formulation: Lyophilized in 25 mM Tris, 150 mM NaCl, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: SerpinA1, PI, A1A, AAT, PI1, A1AT, MGC9222, PRO2275, MGC23330
Gene Symbol: SERPINA1
Molecular Weight: 45.7 kDa
Product Line: Sino Biological®
Product Size: 5 x 5 µg
Protein Family: Proteases Peptidases & Inhibitors
Protein Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
Protein Subtype: Proteinase Inhibitor
Protein Tag or Fusion: His Tag
Purity: ≥ 90% by SDS-PAGE
Research Category: Other Research
Species: Human

Contents & storage

Includes 5 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols