DMEM, high glucose, pyruvate, no glutamine

Description

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

cGMP manufacturing and quality system
DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable DMEM product made in our Scotland facility (21969-035). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Specifications

HEPES Buffer:	No HEPES
Serum Supplementation:	Standard Serum Supplementation
Shipping Condition:	Room Temperature
Volume (Metric):	500 ml
Product Line:	Gibco™
Form:	Liquid
Glutamine:	No Glutamine
Phenol Red Indicator:	Phenol Red
Green Features:	Sustainable packaging
Quantity:	500mL
Glucose:	High Glucose
Sodium Pyruvate Additive:	Sodium Pyruvate

Contents & storage

Media formulations