DMEM, high glucose, pyruvate, no glutamine
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DMEM, high glucose, pyruvate, no glutamine
Gibco™

DMEM, high glucose, pyruvate, no glutamine

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.Read more
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Catalog NumberQuantity
1031303910 x 500 mL
10313021500 mL
Catalog number 10313039
Price (USD)/ 10 x 500 mL
297.65
Online exclusive
319.00 
Save 21.35 (7%)
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Quantity:
10 x 500 mL
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Price (USD)/ 10 x 500 mL
297.65
Online exclusive
319.00 
Save 21.35 (7%)
Add to cart
DMEM, high glucose, pyruvate, no glutamine
Catalog number10313039
Price (USD)/ 10 x 500 mL
297.65
Online exclusive
319.00 
Save 21.35 (7%)
-
Add to cart

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.


This DMEM is modified as follows:
WithWithout
• High Glucose• L-glutamine
• Phenol Red• HEPES
• Sodium Pyruvate 


The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineHeLa, 293, Cos-7, and PC-12
Cell TypePrimary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells
ClassificationAnimal Origin-free
Concentration1 X
FormLiquid
Product TypeDMEM (Dulbecco's Modified Eagle Medium)
Serum LevelStandard Serum Supplementation
Shelf Life12 Months From Date of Manufacture
SterilitySterile-filtered
With AdditivesHigh Glucose, Phenol Red, Sodium Pyruvate
Without AdditivesNo Glutamine, No HEPES
Green FeaturesSustainable packaging
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Quantity10 x 500 mL
Shipping ConditionRoom Temperature
Unit Size10 x 500 mL
Contents & Storage
Storage conditions: 2°C to 8°C (protect from light)
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture