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MMP9 Recombinant Human Protein, His Tag

Catalog number: 10327H08H25

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Description

Matrix Metallopeptidase 9 (Gelatinase B 92Kda Gelatinase 92Kda Type Iv Collagenase) (MMP9), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in protein studies such as enzymetic analysis and protein-protein interactions. In general, recombinant proteins can also be used as an immunogen, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the pro form of human MMP9 enzyme (NP_004985.2) (Met 1-Asp 707) fused to a C-terminal polyhistidine tag.

Activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2, (AnaSpec, Cat#27076). The specific activity is >800 pmoles/min/ µg .

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by SINO Biological.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: MMP9, CLG4B, GELB, MMP-9
Gene Symbol: MMP9
Molecular Weight: 77.7 kDa
Product Size: 5 x 5 µg
Protein Family: Other Proteins
Protein Subtype: Proteases
Protein Tag or Fusion: His Tag
Purity: ≥ 90% by SDS-PAGE
Research Category: Angiogenesis, Cardiovascular Research, Drug Development, Inflammation
Species: Human
Validated Application: Assay Standard

Contents & storage

Includes 5 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols