CaptureSelect™ LC-kappa (Human) Affinity Ligand

CaptureSelect LC-kappa (Human) Affinity Ligand is a 13-kDa recombinant single domain antibody fragment (VHH affinity ligand) that specifically binds to the constant domain of human kappa light chains. It binds to all human antibody isotypes and subclasses (e.g., IgG1 to 4, IgM, IgA, IgD, and IgE) and Fab fragments thereof that contain a kappa light chain, as well as to recombinant human Fab fragments and/or Fab and Fab2 fragments obtained after papain or pepsin digestion.

As described by Ereño-Orbea et. al. (1), this affinity ligand can be used as a crystallization chaperone to enhance the crystallization probability of human Fab fragments and assist in antibody structure determinations.

Free of animal components

CaptureSelect products contain affinity ligands based on recombinant single-domain antibody fragments (VHH) created by a proprietary technology. The VHH affinity ligand is a 12 to 15 kDa fragment comprising the three complementarity-determining regions (CDRs) that form the antigen-binding domain, efficiently produced in the yeast Saccharomyces cerevisiae by a production process free of any animal components (Animal Origin-Free).

1. Ereño-Orbea J, Sicard T, Cui H, Carson J, Hermans P, Julien JP. Structural Basis of Enhanced Crystallizability Induced by a Molecular Chaperone for Antibody Antigen-Binding Fragments. J Mol Biol. 2018 430(3):322-336.