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ICAM1 Recombinant Human Protein, His Tag

Catalog number: 10346H08H50

 Related applications: Protein Assays and Analysis

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Intercellular adhesion molecule 1 (ICAM1), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain of human ICAM1 (NP_000192.2) (Met 1 - Glu 480) fused to a polyhistidine tag at the C-terminus.

Activity: Measured by the ability of the immobilized protein to support the adhesion of PMA stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to ICAM1 coated plates (12.5 µg/ml, 100 µl/well), approximately 20-40% will adhere specifically

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.


Form: Lyophilized
Purity: ≥ 90% by SDS-PAGE
Species: Human
Expression: Mammalian
Gene Symbol: ICAM1
Gene Aliases: BB2, CD54, P3.58
Product Size: 50 µg
Protein Form: Truncated, Recombinant, Cell Surface Protein
Protein Family: CD Proteins
Endotoxin Level: < 1 EU/µg
Protein Subtype: Intercellular adhesion Molecule
Molecular Weight: 51 kDa
Research Category: Immunology, Virology, Extracellular Matrix & Cell Adhesion, Microbiology
Protein Tag or Fusion: His Tag
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.


Manuals & protocols