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Bac-to-Bac® Baculovirus Expression System

Catalog number: 10359016

Invitrogen™  Related applications: Protein Expression

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Description

The Bac-to-Bac® Baculovirus Expression System enables the efficent production of recombinant baculovirus for expression testing in insect cells. The Bac-to-Bac® System relies on generation of recombinant baculovirus by site-specific transposition in E. colirather than homologous recombination in insect cells (Figure 1). This system features:

Time-saving expression bacmid. With Bac-to-Bac®, the expression cassette of the pFastBac™ vector recombines with the parent bacmid in DH10Bac™ E. coli Competent Cells (included in this system kit) to form an expression bacmid. The bacmid is then transfected into insect cells for production of recombinant baculovirus particles.
Easy colony screening. The parent bacmid in DH10Bac™ E. colicontains a segment of the lacZα gene. The lacZα gene is disrupted upon transposition of the expression cassette into the bacmid allowing for blue/white selection of recombinants. This makes identification of recombinant colonies easy.
Flexibility. The Bac-to-Bac® Baculovirus Expression System comes with a pFastBac™ 1 vector that offers the strong polyhedrin promoter for protein expression and a large multiple cloning site for simplified cloning. Two other vectors are offered separately: pFastBac™ HT vector (Cat. No. 10584-027) for production of histidine-tagged recombinant protein and pFastBac™ Dual vector (Cat. No. 10712-024) for expression of two proteins simultaneously.
Rapid purification of recombinant proteins. The optional pFastBac™ HT vector (Cat. No. 10584-027) produces histidine-tagged recombinant protein. This allows rapid protein purification with nickel-chelating resin.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: Polyhedrin
Vector Type: pFastBac
Product Size: 1 kit
Cloning Method: Restriction Enzyme ⁄ MCS
Protein Tag or Fusion: Untagged

Contents & storage

Each Bac-to-Bac® Baculovirus Expression System includes 10 µg of pFastBac™1 vector, 0.5 ml MAX Efficiency® DH10Bac™ Chemically Competent Cells, pFastBac™ 1-Gus control vector, and 1 ml of Cellfectin® Reagent.

Store MAX Efficiency® DH10Bac™ Competent Cells at -80°C. Store Cellfectin® Reagent and vectors at +4°C. All components are guaranteed stable for 6 months when properly stored.

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