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Gibco™

MAX Efficiency™ DH10Bac Competent Cells

Catalog number: 10361012
Gibco™

MAX Efficiency™ DH10Bac Competent Cells

Catalog number: 10361012
Catalog Number
10361012
Unit Size
5 x 100 µL
Price (USD)
Price: 614.00
Your Price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
103610125 x 100 µL
Price: 614.00
Your Price:
-
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MAX Efficiency DH10Bac Competent Cells are used for production of recombinant bacmids used in the Bac-to-Bac Baculovirus Expression System. The DH10Bac E. coli strain contains a baculovirus shuttle vector (bacmid) that can recombine with a donor plasmid, pFastBac, to create an expression bacmid containing a cloned gene of interest. Features of MAX Efficiency DH10Bac Competent Cells include:

• Consistent yields of >1 × 108 transformants/µg
• Tolerance of small amounts of undiluted ligation reactions
• Support of efficient blue/white colony screening (Φ80lacZΔM15 marker)

Using MAX Efficiency DH10Bac competent cells
MAX Efficiency DH10Bac Competent Cells can serve as a host for a recombinant pFastBac vector containing a cloned gene of interest. DH10Bac cells harbor a baculovirus shuttle vector (bMON14272) and a helper plasmid (pMON7142), and are capable of supporting site-specific recombination between pFastBac and bMON14272 to generate high molecular weight bacmids that can then be amplified, purified, and used for insect cell transfection and subsequent gene expression. Kanamycin resistance for bacmid selection and maintenance is conferred by bMON14272, and tetracycline resistance by pMON7124.

Genotype:F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ-rpsL nupG/pMON14272/pMON7124
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Competent Cells
Contains F' Episome
Lacks F' Episome
Efficiency
> 1x10^8
Cell Type
Chemically Competent
Improves Plasmid Quality
No
Cloning Methylated DNA
No
Transformation Efficiency Level
Medium Efficiency (10^8-10^9 cfu⁄µg)
Cloning Unstable DNA
Not Suitable for cloning unstable DNA
Bacterial or Yeast Strain
DH10Bac
Blue/White Screening
Yes
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Preparing Unmethylated DNA
Not Suitable for preparing unmethylated DNA
Propagating ccdB Vectors
Not for ccdB vector propagation
Reduces Recombination
No
Shipping Condition
Dry Ice
T1 Phage - Resistant (tonA)
No
Species
E. coli
Format
Tube
Product Line
DH10Bac, MAX Efficiency™
Quantity
5 x 100 μL

Contents & Storage

Contains:
• MAX Efficiency DH10Bac Chemically Competent Cells: 5 vials, 100 µl each (total of 500 µl)
• pUC19 DNA (0.01 µg/ml): 1 vial, 100 µl
• SOC Medium: 2 bottles, 6 ml each

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.