New—ExpiSf™ Expression System for superior protein yields from insect cells. Learn more »
Gibco™

MAX Efficiency™ DH10Bac Competent Cells

Catalog number:  10361012

Related applications:

Protein Expression

| Transformation

Back to top

Description

MAX Efficiency DH10Bac Competent Cells are used for production of recombinant bacmids used in the Bac-to-Bac Baculovirus Expression System. The DH10Bac E. coli strain contains a baculovirus shuttle vector (bacmid) that can recombine with a donor plasmid, pFastBac, to create an expression bacmid containing a cloned gene of interest. Features of MAX Efficiency DH10Bac Competent Cells include:

• Consistent yields of >1 × 108 transformants/µg
• Tolerance of small amounts of undiluted ligation reactions
• Support of efficient blue/white colony screening (Φ80lacZΔM15 marker)

Using MAX Efficiency DH10Bac competent cells
MAX Efficiency DH10Bac Competent Cells can serve as a host for a recombinant pFastBac vector containing a cloned gene of interest. DH10Bac cells harbor a baculovirus shuttle vector (bMON14272) and a helper plasmid (pMON7142), and are capable of supporting site-specific recombination between pFastBac and bMON14272 to generate high molecular weight bacmids that can then be amplified, purified, and used for insect cell transfection and subsequent gene expression. Kanamycin resistance for bacmid selection and maintenance is conferred by bMON14272, and tetracycline resistance by pMON7124.

Genotype:F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ-rpsL nupG/pMON14272/pMON7124
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Bacterial or Yeast Strain: DH10Bac
Blue/White Screening: Yes
Cloning Methylated DNA: No
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Competent Cell Type: Chemically Competent
F'episome (to make ssDNA): Lacks F' Episome
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Product Line: DH10Bac, MAX Efficiency™
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: No
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): No
Transformation Efficiency: > 1x10^8
Transformation Efficiency Level: Medium Efficiency (10^8-10^9 cfu⁄µg)
Format: Tube(s)
Improves Plasmid Quality: No
Species: E. coli

Contents & storage

Contains:
• MAX Efficiency DH10Bac Chemically Competent Cells: 5 vials, 100 µl each (total of 500 µl)
• pUC19 DNA (0.01 µg/ml): 1 vial, 100 µl
• SOC Medium: 2 bottles, 6 ml each

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.