MAX Efficiency™ DH10Bac Competent Cells
New—ExpiSf™ Expression System for superior protein yields from insect cells. Learn more »
MAX Efficiency™ DH10Bac Competent Cells
Gibco™

MAX Efficiency™ DH10Bac Competent Cells

MAX Efficiency DH10Bac Competent Cells are used for production of recombinant bacmids used in the Bac-to-Bac Baculovirus Expression System. TheRead more
Have Questions?
Catalog NumberQuantity
103610125 x 100 μL
Catalog number 10361012
Price (USD)/ Each
709.65
Online Exclusive
762.00
Save 52.35 (7%)
Add to cart
Quantity:
5 x 100 μL
Price (USD)/ Each
709.65
Online Exclusive
762.00
Save 52.35 (7%)
Add to cart
MAX Efficiency DH10Bac Competent Cells are used for production of recombinant bacmids used in the Bac-to-Bac Baculovirus Expression System. The DH10Bac E. coli strain contains a baculovirus shuttle vector (bacmid) that can recombine with a donor plasmid, pFastBac, to create an expression bacmid containing a cloned gene of interest. Features of MAX Efficiency DH10Bac Competent Cells include:

• Consistent yields of >1 × 108 transformants/μg
• Tolerance of small amounts of undiluted ligation reactions
• Support of efficient blue/white colony screening (Φ80lacZΔM15 marker)

Using MAX Efficiency DH10Bac competent cells
MAX Efficiency DH10Bac Competent Cells can serve as a host for a recombinant pFastBac vector containing a cloned gene of interest. DH10Bac cells harbor a baculovirus shuttle vector (bMON14272) and a helper plasmid (pMON7142), and are capable of supporting site-specific recombination between pFastBac and bMON14272 to generate high molecular weight bacmids that can then be amplified, purified, and used for insect cell transfection and subsequent gene expression. Kanamycin resistance for bacmid selection and maintenance is conferred by bMON14272, and tetracycline resistance by pMON7124.

Genotype:F-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ-rpsL nupG/pMON14272/pMON7124
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Bacterial or Yeast StrainDH10Bac
Blue/White ScreeningYes
Cell TypeChemically Competent
Cloning Methylated DNANo
Cloning Unstable DNANot Suitable for cloning unstable DNA
Contains F' EpisomeLacks F' Episome
Efficiency> 1x10^8
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Improves Plasmid QualityNo
Preparing Unmethylated DNANot Suitable for preparing unmethylated DNA
Product LineDH10Bac, MAX Efficiency™
Product TypeCompetent Cells
Propagating ccdB VectorsNot for ccdB vector propagation
Quantity5 x 100 μL
Reduces RecombinationNo
Shipping ConditionDry Ice
T1 Phage - Resistant (tonA)No
Transformation Efficiency LevelMedium Efficiency (10^8-10^9 cfu⁄μg)
FormatTube
SpeciesE. coli
Unit SizeEach
Contents & Storage
Contains:
• MAX Efficiency DH10Bac Chemically Competent Cells: 5 vials, 100 μl each (total of 500 μl)
• pUC19 DNA (0.01 μg/ml): 1 vial, 100 μl
• SOC Medium: 2 bottles, 6 ml each

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.