Gibco™

MCDB 131 Medium, no glutamine

Catalog number: 10372019
Have Questions?
Gibco™

MCDB 131 Medium, no glutamine

Catalog number: 10372019
Have Questions?
Catalog Number
10372019
Unit Size
500 mL
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
10372019500 mL
-
Product Overview
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MCDB 131 Medium was originally developed by Knedler and Ham as reduced serum-supplemented medium for the culture of human microvascular endothelial cells (HMVEC). MCDB 131 Medium is also used with other cell types, including human omental microvascular cells, hepatocytes, myocytes, and smooth muscle cells.

This MCDB 131 Medium is manufactured as follows:
WithWithout
• Phenol Red• L-glutamine
• Sodium pyruvate• HEPES

The complete formulation is available.

Using MCDB 131
MCDB 131 Medium contains many components not found in traditional basal media, such as trace elements, putrescine, adenine, thymidine, and higher levels of some amino acids and vitamins. These additions allow the medium to be supplemented with very low levels of serum or defined components. MCDB 131 Medium contains no proteins or growth factors, and is often supplemented with EGF, hydrocortisone, glutamine, and low levels of Fetal Bovine Serum (FBS). The FBS concentration must be optimized for each cell type. MCDB 131 Medium uses a sodium bicarbonate buffer system (1.176 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Dual-site cGMP manufacturing
For supply chain continuity, we manufacture MCDB 131 at two separate facilities, located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP-manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type
Human Microvascular Endothelial Cells (HMVEC), Human Omental Microvascular Cells, Hepatocytes, Myocytes, Smooth Muscle Cells
Concentration
1 X
Culture Type
Mammalian Cell Culture
Form
Liquid
Product Type
MCDB 131 Medium
Shelf Life
12 Months From Date of Manufacture
Sterility
Sterile-filtered
With Additives
Low Glucose, Phenol Red, Sodium Pyruvate
Without Additives
No Glutamine, No HEPES
Green Features
Sustainable packaging
Product Line
Gibco™
Quantity
500 mL
Shipping Condition
Room Temperature

Contents & Storage

Storage conditions: 2–8°C (protect from light)
Shelf life: 12 months from date of manufacture

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