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FCGR2A (CD32A) Recombinant Human Protein, His.AVI Tag, Biotinylated

Catalog number: 10374H27H1B25

 Related applications: Protein Assays and Analysis

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Description

Fc Gamma Receptor II-A (FCGR2A), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Met 210) of human FCGR2A (AAA35827.1) (167Arg/His) fused to a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme).

Activity:
1.Measured by its binding ability in a functional ELISA. Immobilized human IgG2 at 10 µg/ml (100 µl/well) can bind biotinylated human FCGR2A with a linear ranger of 0.16-0.8 µg/ml.

2. Using the Octet RED System. The recombinant human FCGR2A and BirA bound to human IgG. The affinity constant (Kd) was 12 nM.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: CD Proteins
Research Category: Immunology
Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: RP11-5K23.6, CD32, CD32A, CDw32, FCG2, FCGR2, FCGR2A, FCGR2A1, FcGR, IGFR2, MGC23887
Gene Symbol: FCGR2A
Molecular Weight: 23.6 kDa
Product Line: Sino Biological®
Product Size: 5 x 5 µg
Protein Name: Fc gamma receptor II-a
Protein Subtype: Fc Receptors
Protein Tag or Fusion: AVI Tag, His Tag
Purity: ≥ 90% by SDS-PAGE
Species: Human
Validated Application: Assay Standard

Contents & storage

Includes 5 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols