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FCGR3A (CD16a), 176 Phe/Val Recombinant Human Protein, His.AVI Tag, Biotinylated

Catalog number: 10389H27H1B5

 Related applications: Protein Assays and Analysis

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Description

Fc gamma receptor III-a (FCGR3A/CD16a), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in studying protein-protein interactions, and cell biology research applications. It can also be used as a protein standard.

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Gln 208) of human FCGR3A (P08637-1), containing the 176 Phe/Val mutation, fused to a polyhistidine and AVI tags at the C-terminus. This protein is biotinylated.

Activity: Measured by its binding ability in a functional ELISA. Immobilized Protein A at 0.5 µg/well can bind human IgG1 with a linear range of 0.078-0.625 µg/mlwith 3 mg/mlof biotinylated human FCGR3A.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: CD Proteins, Growth Factors & Receptors
Research Category: Immunology, Inflammation
Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: RP11-5K23.1, CD16, CD16A, FCG3, FCGR3, FCGRIII, FCR-10, FCRIII, FCRIIIA, IGFR3
Gene ID: 2214
Gene Symbol: FCGR3A
Molecular Weight: 25.6 kDa
Product Line: Sino Biological®
Product Size: 5 µg
Protein Form: Cell Surface Protein, Recombinant, Truncated
Protein Name: Fc fragment of IgG, low affinity IIIa, receptor (CD16a)
Protein Subtype: Fc Receptors, Glycoproteins
Protein Tag or Fusion: AVI Tag, His Tag
Purity: ≥ 90% by SDS-PAGE
Species: Human
Validated Application: Assay Standard
Shipping Condition: Room Temperature

Contents & storage

Store at -20 to -70 °C

Documents

Manuals & protocols