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TNFSF10 Recombinant Human Protein

Catalog number: 10409HNAE25

 Related applications: Protein Assays and Analysis

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Tumor Necrosis (Ligand) Receptor Superfamily Member 10 (TNFSF10), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications

This recombinant protein was expressed from a DNA sequence encoding the human TNFSF10 (NP_003801.1) (Val 114-Gly 281) fused to an initial Met.

1. Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 4-8 ng/ml.

2. Measured in a cytotoxicity assay using NCI-H460 cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.2-0.8 ng/ml.

Formulation: Lyophilized in 40 mM Tris 0.3M NaCl PH7.05% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.
For Research Use Only. Not for use in diagnostic procedures.


Form: Lyophilized
Purity: ≥ 95% by SDS-PAGE
Species: Human
Expression: E. coli
Gene Symbol: TNFSF10
Gene Aliases: APO2L, Apo-2L, CD253, TL2, TRAIL
Product Size: 5 x 5 µg
Protein Family: Cytokines & Receptors, CD Proteins
Protein Subtype: TNF (Tumor Necrosis Factor)
Molecular Weight: 19.6 kDa
Research Category: Apoptosis, Oncology, Signal Transduction
Protein Tag or Fusion: Untagged
Validated Application: Assay Standard

Contents & storage

Includes 5 vial of lyophilized protein. Store at -20 °C.


Manuals & protocols

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