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TNFRSF1B Recombinant Human Protein, hIgG1-Fc.His Tag

Catalog number: 10417H03H25

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Tumor Necrosis Factor Receptor Superfamily Member 1B (TNFRSF1B), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications

This recombinant protein was expressed from a DNA sequence encoding the extracellular domain (Met 1-Asp 257) of human TNFRSF1B (NP_001057.1) fused to the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.

Activity: Measured by its ability to inhibit the TNF-alfa mediated cytotoxicity in the L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 4-16 ng/ml in the presence of 0.25 ng/ml recombinant human TNF-alfa.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.
For Research Use Only. Not for use in diagnostic procedures.


Form: Lyophilized
Purity: ≥ 95% by SDS-PAGE
Species: Human
Expression: Mammalian
Gene Symbol: TNFRSF1B
Product Size: 5 x 5 µg
Protein Family: Cytokines & Receptors, CD Proteins
Endotoxin Level: < 1 EU/µg
Protein Subtype: TNF Receptors
Molecular Weight: 53 kDa
Research Category: Cardiovascular Research, Inflammation
Protein Tag or Fusion: His Tag, IgG1 Fc Tag
Validated Application: Assay Standard

Contents & storage

Includes 5 vial of lyophilized protein. Store at -20 °C.


Manuals & protocols

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