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VTN Recombinant Human Protein, His Tag

Catalog number: 10424H07H5

 Related applications: Protein Assays and Analysis

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Vitronectin (VTN), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.

This recombinant protein was expressed from a DNA sequence encoding the mature form of human VNT (NP_000629.3) (Asp 20-Leu 478) fused to a polyhistidine tag at N-terminus.

Activity: Measured by the ability of the immobilized protein to support the adhesion of DU145 human prostate carcinoma cells.
When cells are added to vitronectin coated plates (100 µl/well of a 5 µg/mlsolution), approximately 80-90% will adhere specifically after 30 minutes at 37°C

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.

This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.


Species: Human
Protein Subtype: Vitronectin
Validated Application: Assay Standard
Research Category: Oncology, Extracellular Matrix & Cell Adhesion, Angiogenesis
Protein Form: Full Length, Recombinant
Form: Lyophilized
Purity: ≥ 85% by SDS-PAGE
Expression: Mammalian
Gene Symbol: VTN
Gene Aliases: VTN, V75, VN, VNT
Product Size: 5 µg
Protein Family: Other Proteins
Endotoxin Level: < 1 EU/µg
Molecular Weight: 65 kDa
Protein Tag or Fusion: His Tag

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.


Manuals & protocols