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VTN Recombinant Human Protein, His Tag

Catalog number: 10424H07H5

 Related applications: Protein Assays and Analysis

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Description

Vitronectin (VTN), recombinant human protein is supplied as a lyophilized powder. It is suitable for use in analysis of protein structure, and performing cell based assays. It can also be used as an immunogen, as a protein standard, or in other research applications.

This recombinant protein was expressed from a DNA sequence encoding the mature form of human VNT (NP_000629.3) (Asp 20-Leu 478) fused to a polyhistidine tag at N-terminus.

Activity: Measured by the ability of the immobilized protein to support the adhesion of DU145 human prostate carcinoma cells.
When cells are added to vitronectin coated plates (100 µl/well of a 5 µg/mlsolution), approximately 80-90% will adhere specifically after 30 minutes at 37°C
.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Protein Family: Other Proteins
Research Category: Angiogenesis, Extracellular Matrix & Cell Adhesion, Oncology
Species: Human
Protein Subtype: Vitronectin
Validated Application: Assay Standard
Protein Form: Full Length, Recombinant
Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: VTN, V75, VN, VNT
Gene Symbol: VTN
Molecular Weight: 65 kDa
Product Line: Sino Biological®
Product Size: 5 µg
Protein Name: vitronectin
Protein Tag or Fusion: His Tag
Purity: ≥ 85% by SDS-PAGE

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols