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IGFBP3 Recombinant Human Protein, His Tag

Catalog number: 10430H07H50

 Related applications: Protein Assays and Analysis

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Description

Insulin-like growth factor binding protein 3 (IGFBP3), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins are suitable for use in studying protein-protein interactions, as a protein standard, or in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the mature form of human IGFBP3 (NP_000589.2), residues 28 to 291), fused to a polyhistidine tag at the N-terminus.

Activity: Measured by its binding ability in a functional ELISA.
1. Immobilized human IGF2 at 10 µg/ml(100 µl/well) can biotinylated human IGFBP3-His. The ED50 of can biotinylated human IGFBP3-His is 18 ng/ml. 2. Immobilized human IGF1 at 10 µg/ml(100 µl/well) can biotinylated human IGFBP3-His. The ED50 of can biotinylated human IGFBP3-His is 24 ng/ml.

Formulation: Lyophilized in PBS, pH 7.4, 5% Mannitol, 5% Trehalose, and 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Research Category: Apoptosis, Development, Neurobiology, Oncology
Endotoxin Level: < 1 EU/µg
Expression: Mammalian
Form: Lyophilized
Gene Aliases: IGFBP3, tcag7.703, BP-53, IBP3
Gene ID: 3486
Gene Symbol: IGFBP3
Molecular Weight: 31 kDa
Product Line: Sino Biological®
Product Size: 50 µg
Protein Family: Growth Factors & Receptors
Protein Form: Recombinant
Protein Name: Insulin-like growth factor binding protein 3
Protein Subtype: IGFs (Insulin-Like Growth Factors)
Protein Tag or Fusion: His Tag
Purity: ≥ 85% by SDS-PAGE
Species: Human
Validated Application: Assay Standard
Shipping Condition: Room Temperature

Contents & storage

Store at -20 to -70 °C

Documents

Manuals & protocols

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