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Embryonic stem-cell FBS, qualified, USDA-approved regions
Gibco™

Embryonic stem-cell FBS, qualified, USDA-approved regions

Embryonic stem-cell FBS, qualified, USDA-approved regions is US certified performance plus sera for broad range of cell types, especially sensitive cell lines.
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Catalog NumberQuantityFormat
1043900150 mLOne Shot
10439024500 mLBottle
Catalog number 10439001
Price (USD)
126.00
Each
Quantity:
50 mL
Format:
One Shot
Price (USD)
126.00
Each

Gibco™ fetal bovine sera offers excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements

  • Sera Category: Specialty
  • Origin: Countries meeting USDA importation requirements
  • Endotoxin level: ≤50 EU/mL (levels routinely ≤10 EU/mL)
  • Hemoglobin level: ≤25 mg/dL

Specially tested for the ability to sustain undifferentiated cellular morphology of embryonic stem cells.

For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.

Specifications
Donor HerdNo
Endotoxin Concentration<50 EU/mL
Hemoglobin Concentration≤25 mg/dL
Product LineOne Shot
Purity or Quality GradeES Cell-Qualified
Shipping ConditionFrozen
SpeciesCattle/Bovine
Tested ForBovine Respiratory Syncytial Virus FA, Cytopathic Agents, Hemadsorbing Agents, Reovirus FA, Rabies FA, Hemoglobin (ng/dL), Blue Tongue Virus, Bovine Adenovirus FA, Bovine Parvovirus FA, Osmolality, Sp2 Performance Assay, Electrophoretic Pattern, Total Protein, Endotoxin (EU/mL), Mycoplasma, Sterility Testing (Bacteria, Fungi)
AgeFetal
Country of OriginMexico
FormLiquid
FormatOne Shot
Product TypeFetal Bovine Serum
Quantity50 mL
Serum TreatmentStandard (Sterile-filtered)
SterilitySterile
Unit SizeEach
Contents & Storage
Storage conditions: ≤-10°C
Shipping conditions: Frozen

Frequently asked questions (FAQs)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

Do I need to heat-inactivate Embryonic Stem-Cell FBS, qualified?

The material is not heat-inactivated. We would only recommend heat-inactivating it if your application specifically requires it. We recommend using heat-inactivated serum when working with immune type cells or for immunological applications.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.