DMRIE-C Transfection Reagent

Citations & References (18)

Invitrogen™

DMRIE-C Transfection Reagent

DMRIE-C Transfection Reagent is suitable for transfecting DNA and RNA into eukaryotic cells, and is particularly effective for transfecting suspensionRead more

Catalog Number	Quantity
10459014	1 mL

Catalog number 10459014

Price (MXN)

Quantity:

DMRIE-C Transfection Reagent is suitable for transfecting DNA and RNA into eukaryotic cells, and is particularly effective for transfecting suspension cells (e.g., Jurkat) and other lymphoid-derived cell lines. Refer to the Cell Lines database at www.invitrogen.com for a list of cell types successfully transfected. DMRIE-C can also be used for in vivo delivery of DNA. DMRIE-C is a 1:1 (M/M) liposome formulation of the cationic lipid DMRIE (1,2-dimyristyloxy-propyl-3-dimethyl-hydroxy ethyl ammonium bromide) and cholesterol in membrane-filtered water.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Application)Transfection

High-throughput CompatibilityNot High-throughput Compatible (Manual)

Product TypeTransfection Reagent

Quantity1 mL

Serum CompatibleNo

Shipping ConditionWet Ice

Cell TypeEstablished Cell Lines

Format6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks

Sample TypePlasmid DNA

Transfection TechniqueLipid-based Transfection

Unit SizeEach

Contents & Storage

Contains one tube (1 ml) DMRIE-C Transfection Reagent. Store at 4°C. Do not freeze.

Frequently asked questions (FAQs)

I accidentally left my lipid reagent at room temperature. Can I still use it?

Yes, all of our lipid transfection reagents are stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

Do you offer a reagent for the transfection of suspension cells (e.g., Jurkat cells)?

Lipofectamine 3000 Reagent may be used with Jurkat cells but the highest transfection efficiency (~98%) can be achieved using the Neon Transfection System.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

What is the difference between reverse transfection and forward transfection? What should I use?

In forward transfection, cells are seeded to appropriate confluence or cell density in wells or dishes, and the lipid-DNA complexes are added the next day. In reverse transfection, the transfection complexes are prepared inside the wells, after which cells and medium are added. Reverse transfection is faster to perform than forward transfection, and is the method of choice for high-throughput transfection. For non-high-throughput transfections, generally forward transfections have better efficiency for most cell types.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Is there a place where I can find references from other researchers who have used your transfection reagents?

Visit the product page for each reagent type and you will see a list of references at the bottom of the page. A table that lists specific cell line references is also accessible. We also recommend www.highwire.org as a search engine to find a large selection of up-to-date research articles using our transfection products. Simply include the name of the transfection reagent and your cell line/application of interest in your search criteria.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I use antibiotics in the medium during transfection?

Antibiotics can be used in the medium for culturing of cell lines. However, we do not recommend using antibiotics in the transfection medium unless previously tested in the cell type and payload being transfected. This is because presence of antibiotics during transfection may adversely affect transfection efficiency (i.e., positively charged antibiotics binding to the DNA being transfected) and overall health of cells being transfected.

For stable transfection, we recommend waiting wait 24-48 hrs after transfection before adding selected antibiotics.

Find additional tips, troubleshooting help, and resources within ourTransfection Basics Support Center.

View all DMRIE-C Transfection Reagent FAQs

Citations & References (18)

Citations & References

Abstract

Induction of bacterial lipoprotein tolerance is associated with suppression of toll-like receptor 2 expression.

Authors:Wang JH, Doyle M, Manning BJ, Di Wu Q, Blankson S, Redmond HP.

Journal:J Biol Chem

PubMed ID:12133836

'Tolerance to bacterial cell wall components including lipopolysaccharide (LPS) may represent an essential regulatory mechanism during bacterial infection. Two members of the Toll-like receptor (TLR) family, TLR2 and TLR4, recognize the specific pattern of bacterial cell wall components. TLR4 has been found to be responsible for LPS tolerance. However, the ... More

Safety and short-term toxicity of a novel cationic lipid formulation for human gene therapy.

Authors:San H, Yang ZY, Pompili VJ, Jaffe ML, Plautz GE, Xu L, Felgner JH, Wheeler CJ, Felgner PL, Gao X

Journal:Hum Gene Ther

PubMed ID:8186291

'Among the potential nonviral vectors for human gene therapy are DNA-liposome complexes. In a recent clinical study, this delivery system has been utilized. In this report, a novel cationic lipid, dimyristyloxypropyl-3-dimethyl-hydroxyethyl ammonium (DMRIE), has been substituted into the DNA-liposome complex with dioleoyl phosphatidylethanolamine (DOPE), which both improves transfection efficiencies and ... More

Nitrosative Stress-induced Apoptosis through Inhibition of NF-kappa B.

Authors: Marshall Harvey E; Stamler Jonathan S;

Journal:J Biol Chem

PubMed ID:12091382

'Nitrosative stress produced by cytokines predisposes to apoptotic cell death. However, the molecular mechanism by which this occurs is not well understood. We have shown previously that nitric oxide (NO) regulates the activity of the anti-apoptotic transcription factor NF-kappaB. Here we demonstrate that the inhibition of NF-kappaB by NO sensitizes ... More

Evaluation of cationic liposome suitable for gene transfer into pregnant animals.

Authors:Ochiya T, Takahama Y, Baba-Toriyama H, Tsukamoto M, Yasuda Y, Kikuchi H, Terada M

Journal:Biochem Biophys Res Commun

PubMed ID:10329392

'Cationic liposome-mediated in vivo gene transfer represents a promising approach for somatic gene therapy. To assess the most suitable liposome for gene delivery into a wide range of organs and fetuses in mice, we have explored several types of cationic liposomes conjugated with plasmid DNA carrying the beta-galactosidase gene through ... More

BiZyme: a novel fusion protein-mediating selection of vaccinia virus recombinants by fluorescence and antibiotic resistance.

Authors: Hansen Scott G; Cope Torrey A; Hruby Dennis E;

Journal:Biotechniques

PubMed ID:12019792

'Recombinant vaccinia virus is a useful and powerful tool for the expression and study of foreign genes. Methods that are currently available for the selection of vaccinia virus recombinants include the restoration of viral plaque-forming phenotype, the replication of viral DNA in the presence of BUdR or mycophenolic acid, and ... More

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