'Development of skeletal cartilage is characterized with coupling growth arrest and cell differentiation. Here, to understand the cyclin-dependent kinase inhibitors involved in the progression of chondrogenic differentiation, we examined changes in the expression levels of cyclin-dependent kinase inhibitor members using mouse ATDC5 prechondrocytes as a widely used in vitro model ... More
A new type of high affinity folic acid transporter in the protozoan parasite Leishmania and deletion of its gene in methotrexate-resistant cells.
AuthorsRichard D, Kundig C, Ouellette M.
JournalJ Biol Chem
PubMed ID12023977
'The protozoan parasite Leishmania is a folate auxotroph and thus depends on the uptake of folate from the environment to meet its folate requirement. We show here that Leishmania contains several putative pteridine transporter genes. Some of these genes are deleted in methotrexate-resistant Leishmania cells where there is no measurable ... More
Isolation of high-molecular-weight genomic DNA from intact biohazardous mammalian tissues.
AuthorsLiska V; Ruprecht R M;
JournalBiotechniques
PubMed ID9894593
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DNA extraction from fixed cytogenetic cell suspensions.
AuthorsAmorim MR, Vargas FR, Llerena JC, Pombo-de-Oliveira MS
JournalGenet Mol Res
PubMed ID17985302
We developed a procedure for DNA extraction from small volumes of fixed cell suspensions previously prepared for conventional cytogenetic analysis. Good quality DNA was isolated with a fast and simple protocol using DNAzol reagent. This provided suitable DNA for various types of molecular analyses, including polymerase chain reaction, restriction fragment ... More
Rapid and efficient extraction of genomic DNA from different phytopathogenic fungi using DNAzol reagent.
AuthorsGuo JR, Schnieder F, Abd-Elsalam KA, Verreet JA
JournalBiotechnol Lett
PubMed ID15685411
A modified procedure using the commercial DNAzol reagent was successfully applied to extract genomic DNA from 25 fungal species. The DNA yield varied from 306 to 1,927 microg g(-1) dry mycelia and the A(260)/A(280) ratio from 1.59 to 1.93. Compared with the method of J.L. Cenis (Nucleic Acids Res. 1992, ... More
HOXB4-Induced Expansion of Adult Hematopoietic Stem Cells Ex Vivo.
Authors Antonchuk Jennifer; Sauvageau Guy; Humphries R Keith;
JournalCell
PubMed ID11955445
Hox transcription factors have emerged as important regulators of primitive hematopoietic cell proliferation and differentiation. In particular, HOXB4 appears to be a strong positive regulator of hematopoietic stem cell (HSC) self-renewal. Here we demonstrate the potency of HOXB4 to enable high-level ex vivo HSC expansion. Cultures of nontransduced or GFP-transduced ... More
Chromosomal assignment and deletion mapping of barley EST markers.
AuthorsNasuda S, Kikkawa Y, Ashida T, Islam AK, Sato K, Endo TR
JournalGenes Genet Syst
PubMed ID16394587
From about 10000 PCR-based EST markers of barley we chose 1421 EST markers that were demonstrated to be amplified differently by PCR between wheat (Triticum aestivum cv. Chinese Spring) and barley (Hordeum vulgare cv. Betzes). We assigned them to the seven barley chromosomes (1H to 7H) by PCR analysis using ... More
Analysis of a truncated form of cathepsin H in human prostate tumor cells.
Increased expression of proteases has been correlated with the malignant progression of a variety of tumors. We found a significant increase in cathepsin H expression in high-grade prostatic intraepithelial neoplasia and carcinoma of the prostate. Two forms of cathepsin H, the full-length form (CTSH) and a truncated form with a ... More
A UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase is essential for viability in Drosophila melanogaster.
Authors Ten Hagen Kelly G; Tran Duy T;
JournalJ Biol Chem
PubMed ID11925446
We report the first demonstration that the activity of a member of the UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase gene family is necessary for viability in Drosophila melanogaster. Expression of the wild-type recombinant pgant35A gene in COS7 cells resulted in in vitro activity against peptide and glycopeptide substrates, demonstrating that this gene encodes a ... More
Regulation of DNA methylation in human breast cancer. Effect on the urokinase-type plasminogen activator gene production and tumor invasion.
Urokinase-type plasminogen activator (uPA) is a member of the serine protease family and can break down various components of the extracellular matrix to promote growth, invasion, and metastasis of several malignancies including breast cancer. In the current study we examined the role that the DNA methylation machinery might be playing ... More