DNAzol® Reagent, for isolation of genomic DNA from solid and liquid samples

Catalog number: 10503027

Invitrogen™  Related applications: DNA Extraction

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Description

DNAzol® Reagent is a complete and ready-to-use organic reagent for the isolation of genomic DNA from solid and liquid samples of animal, plant, yeast, and bacterial origin. The DNAzol® Reagent procedure can be completed in typically 10–30 minutes with DNA recoveries of 70–100%. DNAzol® Reagent is:

Versatile—can use with a broad spectrum of sample materials
Efficient—rapid isolation and high recovery of genomic DNA

Efficient isolation of genomic DNA from a variety of sample types
The DNAzol® Reagent procedure uses a novel guanidine-detergent lysing solution that permits selective precipitation of DNA from cell lysate. 1 mL of DNAzol® Reagent can be used to isolate genomic DNA from 1–3 x 107 cells, from 0.1 mL of whole blood, or from 25–50 mg tissue.

Rapid isolation and high recovery of genomic DNA
The DNAzol® Reagent combines reliability, efficiency, and simplicity in its DNA isolation protocol. During isolation, a biological sample is lysed (or homogenized) in DNAzol® Reagent, and the genomic DNA is then precipitated from the lysate with ethanol. Following an ethanol wash, DNA may be solubilized in either water or 8 mM NaOH. The entire procedure can be completed in 10–30 min with DNA recovery of 70–100%.

Isolated DNA can be used for a number of downstream applications
Genomic DNA extracted using the DNAzol® procedure is suitable for a variety of applications, including Southern blotting, cloning, PCR, restriction endonuclease digestion, and dot blot hybridization.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Cells, Tissue
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Downstream Application: PCR, Sequencing, Cloning, Southern Blotting
Product Size: 100 mL
Final Product: Genomic DNA
Isolation Technology: Organic Extraction
Shipping Condition: Room Temperature

Contents & storage

Contents: 1 bottle (100 ml)
Store at 15°C to 30°C

Documents

Manuals & protocols

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