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Invitrogen™

Terminal Deoxynucleotidyl Transferase, recombinant

Catalog number: 10533065
Invitrogen™

Terminal Deoxynucleotidyl Transferase, recombinant

Catalog number: 10533065
Catalog Number
10533065
also known as 10533-065
Unit Size
500 units
Price (USD)
Price: 274.00
Your price:
Availability
-
Quantity
Catalog Number
10533073
also known as 10533-073
Unit Size
3 x 500 units
Price (USD)
Price: 586.00
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
10533065
also known as 10533-065
500 units
Price: 274.00
Your price:
-
10533073
also known as 10533-073
3 x 500 units
Price: 586.00
Your price:
-
Product Overview
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Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is a DNA polymerase that catalyzes the addition of deoxynucleotides to the 3fi hydroxyl terminus of DNA. A TdT Technical Bulletin is available.

Applications:
Homopolymer tailing of vector and insert for cloning. Labeling oligonucleotides with biotin (1,2), 32P- or 35S-label (3), or in apoptosis (TUNEL) (4,5).

Source:
Purified from E. coli clone of calf thymus TdT.

Performance and Quality Testing:
Endonuclease, 3´ and 5´ exodeoxyribonuclease, and levels of incorporation tested.

Unit Definition:
One unit incorporates 1 nmol dATP into acid-precipitable material in 1 h at 37°C, using d(pA)50 as a primer.

Hazard Warning:
Toxic; potassium cacodylate contained in reaction buffer. Also contains cobalt chloride, a highly toxic chemical.
See MSDS.

Unit Reaction Conditions:
0.2 M potassium cacodylate (pH 7.2), 10 mM MgO4 C4 H6 , 1 mM 2-mercaptoethanol, 0.5 mg/ml BSA,
100 flM d(pA)50, 1 mM [3H]dATP, and enzyme in 0.15 ml for 1 h at 37°C.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Shipping Condition
Approved for shipment on Wet or Dry Ice
Enzyme
Terminal Deoxynucleotidyl Transferase
Compatible Buffer
5X Buffer, Reaction Buffer
Quantity
500 U
Product Type
TdT

Contents & Storage

Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is supplied with vial of 5X buffer [500 mM potassium cacodylate (pH 7.2), 10 mM CoCl2 , 1 mM DTT]. Store at -20°C.

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