This product is manufactured in our Grand Island, US facility and is intended for sale in North America, Latin America, and the Asia/Pacific region—higher freight charges will apply to orders shipped elsewhere. An equivalent product, manufactured in Paisley, Scotland, is recommended for our customers in Europe, Africa, and the Middle East. Click to view.
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Gibco™
DMEM, high glucose, GlutaMAX™ Supplement, HEPES
Description
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.This DMEM is modified as follows:
| With | Without |
| • High Glucose | • Sodium Pyruvate |
| • HEPES | |
| • GlutaMAX™ | |
| • Phenol Red |
The complete formulation is available.
Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
cGMP manufacturing and quality system
DMEM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical DMEM product made in our Scotland facility (32430-100). This facility is also registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standard.
For In Vitro Diagnostic Use. For Research Use Only in China.
Specifications
| Green Features: | Sustainable packaging |
|---|---|
| HEPES Buffer: | HEPES |
| Product Line: | Gibco™, GlutaMAX™ |
| Quantity: | 500mL |
| Serum Supplementation: | Standard Serum Supplementation |
| Shipping Condition: | Room Temperature |
| Volume (Metric): | 500 ml |
| For Use With (Application): | Mammalian cell culture |
| Form: | Liquid |
| Glucose: | High Glucose |
| Glutamine: | GlutaMAX™-I |
| Phenol Red Indicator: | Phenol Red |
| Sodium Pyruvate Additive: | No Sodium Pyruvate |
Contents & storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
