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Invitrogen™

PCR SuperMix

Catalog number:  10572014

 Related applications: Chromatin Biology | PCR

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Description

PCR SuperMix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification. Simply add template and primers, reducing set-up time by half. PCR SuperMix contains Mg2+, dNTPs, and recombinant Taq DNA Polymerase at concentrations sufficient for routine PCR of fragments up to 5 kb. PCR SuperMix is supplied at a 1.1X concentration to allow approximately 10% of the final reaction volume to be used for the addition of primer and template solutions.

For superior PCR performance, we recommend DreamTaq DNA Polymerase.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase: Taq DNA Polymerase
Hot Start: No
Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: Low
Reaction Format: SuperMix or Master Mix
Product Overhang: 3'-A
Reaction Speed: Standard
Final Product Size(s): 5 kb or less
Exonuclease Activity: 5' - 3'
Product Size: 100 reactions
Shipping Condition: Approved for shipment on Wet or Dry Ice

Contents & storage

Contains 4 vials (1.125 ml each) of PCR SuperMix.

Components:
22 mM Tris-HCl (pH 8.4), 55 mM KCl, 1.65 mM Magnesium Chloride, 220 µM dGTP, 220 µM dATP, 220 µM dTTP, 220 µM dCTP, 22 U/ml recombinant Taq DNA Polymerase, and stabilizers.

Documents

Manuals & protocols