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IGF1 Recombinant Human Protein, His.NusA Tag

Catalog number: 10598H24E5

 Related applications: Protein Assays and Analysis

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Description

Insulin-like growth factor 1 (IGF1), recombinant human protein is supplied as a lyophilized powder. In general, recombinant proteins can be used as protein standard and in cell biology research applications.

This recombinant protein was expressed from a DNA sequence encoding the mature form of human IGF1 isoform 1 (P05019-1) (Gly 49-Ala 118) fused to the polyhistidine-NusA tag at the N-terminus.

Activity:
Measured by its binding ability in a functional ELISA. Immobilized human IGF1 at 10 µg/ml(100 µl/well) can bind biotinylated human IGFBP3-His. The EC50 of biotinylated human IGFBP3-His is 24 ng/ml.

Formulation: Lyophilized in 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.4, 5% Mannitol, 5% Trehalose, 0.02% Tween 80.

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/ml or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Expiration Date: Expires one year from date of receipt when stored as instructed.



This protein is manufactured by Sino Biological Inc.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Research Category: DNA Replication & Repair, Development, Immunology
Expression: E. coli
Form: Lyophilized
Gene Aliases: IGF-I, IGF1A, IGFI
Gene Symbol: IGF1
Molecular Weight: 64.8 kDa
Product Line: Sino Biological®
Product Size: 5 µg
Protein Family: Growth Factors & Receptors
Protein Form: Full Length, Recombinant
Protein Name: insulin-like growth factor 1 (somatomedin C)
Protein Subtype: IGFs (Insulin-Like Growth Factors)
Protein Tag or Fusion: His Tag, NusA Tag
Purity: ≥ 90% by SDS-PAGE
Species: Human
Validated Application: Assay Standard

Contents & storage

Includes 1 vial of lyophilized protein. Store at -20 °C.

Documents

Manuals & protocols

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